In Vivo Testing System for Determining the Estrogenic Activity of Endocrine- Disrupting Chemicals (EDCs) in Goldfish (Carassius auratus)
نویسندگان
چکیده
Graduate School of Science and Technology, Environmental Protection center, and Faculty of Environmental Studies, Nagasaki University, 1–14 Bunkyo-machi, Nagasaki 852–8521, Japan, Department of Chemical and Biological Engineering, Ariake National College of Technology, 150 Higashihagiomachi, Omuta 836–8585, Japan, and Faculty of Environmental and Symbiotic Sciences, Prefectural University of Kumamoto, 3–1–100 Tsukide, Kumamoto 862–8502, Japan (Received January 9, 2001; Accepted January 25, 2001)
منابع مشابه
Research Article: A rapid approach to assess estrogenic transcriptional activity of bisphenol A in the liver of goldfish
Bisphenol A (BPA) is a synthetic compound widely used in types of consumer goods and medical tools. It has been shown that BPA acts as an endocrine disruptor chemical casuing negative impact on reproductive functions. The present study used an in vitro approach to assess estrogenic transcriptional activity of BPA as a rapid and sensitive method. The cultured pieces of hepatic tissue of goldfish...
متن کاملVitellogenin assay by enzyme-linked immunosorbent assay (ELISA) as a biomarker of endocrine disruptor chemicals (EDCs) pollution
There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...
متن کاملAssessment of Estrogenic Endocrine-Disrupting Chemical Actions in the Brain Using in Vivo Somatic Gene Transfer
Estrogenic endocrine-disrupting chemicals abnormally stimulate vitellogenin gene expression and production in the liver of many male aquatic vertebrates. However, very few studies demonstrate the effects of estrogenic pollutants on brain function. We have used polyethylenimine-mediated in vivo somatic gene transfer to introduce an estrogen response element-thymidine kinase-luciferase (ERE-TK-LU...
متن کاملThe estrogenic effect of genistein on vitellogenin synthesis in the mature male goldfish (Carassius auratus)
In the present study, the effects of estrogenic genistein or 17β-estradiol (E2) was investigated on vitellogenin synthesis in mature male goldfish. Matures male goldfish received intraperitoneal injections of E2 (10 μg/g body weight), one of two genistein doses (5 μg/g body weight, G5, or 50 μg/g body weight, G50), or the injection vehicle every other day for 10 days during spermiation phase. T...
متن کاملActions of Estrogenic Endocrine Disrupting Chemicals on Human Prostate Stem/Progenitor Cells and Prostate Carcinogenesis
Substantial evidences from epidemiological and animal-based studies indicate that early exposure to endocrine disrupting chemicals (EDCs) during the developmental stage results in a variety of disorders including cancer. Previous studies have demonstrated that early estrogen exposure results in life-long reprogramming of the prostate gland that leads to an increased incidence of prostatic lesio...
متن کامل